While this might not be a complete list, I have learned from my own mistakes the importance of asking questions to the people interviewing you. It's easy for one to fall into working on tasks that weren't what you expected without the right questions. Every job is different and this is just the ones that I think are important.

1.) What are the expected hours of this job?
This question may seem obvious, but sometimes it isn't!
2.) How does [company] handle paid time off and holidays?
This question may seem rude to some people. However, your free time is important. You should know what to expect. An interview is just as much you interviewing the company as the company interviewing you.
3.) How often do you expect me to be on site vs. remote?
This doesn't apply to jobs that can only be done in person. Even if this is listed in the job description, sometimes the hiring managers and the actual managers have different ideas on how they expect the employee to work. It's important to verify for your personal preferences.
4.) What does my day to day in this position look like?
This is one that can easily be reworded depending on what type of field you work in. Different roles even with the same title can be entirely different.
5.) What do you enjoy about working for [company]?
This can spark a conversation. If they don't have much to say, it can be a red flag or a green flag depending on if you are hoping for a company with a work culture of doing things outside of work or not.

Add to these some field specific questions and you'll be all set. Good luck out there with this hell of a job market!